Fig. 6

Cg-induced IL-1β production by macrophages depends on ATP efflux through Panx1 channel. a Peritoneal macrophages were pre-incubated with carbenoxolone (Cbx, 50 uM - 30 min) and then stimulated with Cg (300 μg/ml). At indicated times, the supernatants were collected for IL-1β quantification by Elisa. b Peritoneal macrophages harvested from naive WT or Panx1^−/− mice were stimulated with Cg or medium. At indicated time points, the supernatants were collected for quantification of IL-1β by Elisa. c Peritoneal macrophages harvested from naive WT or Panx1^−/− mice were stimulated with Cg or medium. After 3 h, the Il1b mRNA expression was determined by qPCR. d Representative western blotting for immature (p35) or active form (p17) of IL-1β in the supernatant of Cg-stimulated macrophages (24 h). Macrophages were harvested from WT or Panx1^−/− mice. e Peritoneal macrophages harvested from naive WT or Panx1^−/− mice were stimulated with Cg or medium. After 6 h, the supernatants were collected for quantification of eATP concentration. f Peritoneal macrophages were pre-incubated with carbenoxolone (Cbx, 50 uM - 30 min) and then stimulated with Cg. After 6 h, the supernatants were collected for quantification of eATP concentration. Data are represent the mean ± SD of four independent experiments compared WT vs Knockout/Treatments groups to determine the level of statistical significance (*p < 0.05; **p < 0.01; and ***p < 0.001; ns, not significant)