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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Molecular basis of carrageenan-induced cytokines production in macrophages

Fig. 2

Role of TRIF/Syk/reactive oxygen species on Cg-induced Il1b mRNA. Peritoneal macrophages were stimulated with Cg (300 μg/ml) or medium. a ROS production were quantified in supernatant by the fluorescent probe assay (H2DCFDA) after indicated times. b, c The supernatant were collect after pre-treated cells with N-acetylcysteine (NAC – 3 mM) to quantify Il1b mRNA gene expression by qPCR and IL-1β production by Elisa after Cg (6 h). d Quantification of ROS production by (H2DCFDA probe) in cells from naive WT vs deficient for Trif−/− after Cg (3 h). e, f Pre-treated cells with selective inhibitor of Syk (iSyk 1, 3 μM) and stimulated with Cg (6 h) to quantify IL-1β by ELISA and Il1b mRNA gene expression induced by Cg (3 h), medium or (iSyk 3 μM). g Quantification of ROS production (H2DCFDA probe) stimulated with Cg and iSyk (3 uM) after 3 h. h Western blotting analysis of (pSyk) expression compared cells from WT vs Trif−/−. Data are represent the mean ± SD of four independent experiments compared Control vs WT (Cg) or WT vs Knockout/Treatments groups to determine the level of statistical significance (*p < 0.05; **p < 0.01; and ***p < 0.001; ns, not significant)

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