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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The Role of Immunological Synapse in Predicting the Efficacy of Chimeric Antigen Receptor (CAR) Immunotherapy

Fig. 3

Optimizing CAR design for translational research. Blood is collected by a lab professional into a 10 ml tube with anticoagulant as a regular specimen collection procedure in any certified blood testing laboratory. The tube can be sent directly to the IS testing lab without transferring to a secondary tube. PBMCs can be enriched. The enriched PBMCs are placed in culture and expanded in vitro. The viral vectors containing CAR1, CAR2, CAR3, etc. are added to generate different version of CAR constructs generated by a research laboratory. These different CAR constructs are optimized to enhance the functions of CAR T. However, it is impractical to put every single construct into a preclinical study (e.g., in vivo animal model). The CAR T cells are subjected to IS quality testing using both the lipid bilayer chip and VCP device. IS quality ranking reports can be presented to scientists who can select the best CAR construct for a preclinical animal study, and ultimately, the optimal CAR design for downstream clinical applications. The SPE system can identify the best CAR construct from numerous constructs by screening the IS quality

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