Fig. 3
From: Higher sensitivity of female cells to ethanol: methylation of DNA lowers Cyp2e1, generating more ROS
Aldehyde dehydrogenase isoforms are neither responsive to EtOH nor sex dimorphic, while Cyp2e1 is sex dimorphic and responsive. Expression in ED 10.5 cells was measured by qRT-PCR using specific primers to evaluate baseline expression. Samples were normalized at the RNA and cDNA levels for equal cDNA loading. Experiments were done in triplicate. Average CT values are shown. Ordinate is average CT of fluorescence detection for real-time PCR. ED10.5 cells exposed to 400 μM EtOH, DSF, or both for 24 h. ALDH 1 and 2 do not differ by sex or respond to EtOH, while female cells contain more Cyp2e1 message. Both male cells and female cells respond to EtOH, with the female cells responding more, increasing the differential. DSF, which inhibits aldehyde dehydrogenase, stimulates production of Cyp2e1 message in both sexes, eliminating the difference between the sexes. Asterisks (*) represent P < 0.05, standard deviation shown