Fig. 2
From: Higher sensitivity of female cells to ethanol: methylation of DNA lowers Cyp2e1, generating more ROS
Expression Profiles for Alcohol Dehydrogenase Isoforms are not sex specific. Expression was measured by qRT-PCR using specific primers to evaluate their baseline expression. ED10.5 mixed cells. Samples were normalized at the RNA and cDNA levels for equal cDNA loading. Experiments were done in triplicate. Average CT values are shown. Ordinate is average CT of fluorescence detection for real-time PCR. ED10.5 cells exposed to 400 μM EtOH, DSF, or both for 24 h or. ADH 1B, 5, and 6 are neither responsive to EtOH nor sex dimorphic; ADH 4 and 7 are responsive to EtOH but not sex dimorphic