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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: SRSF1 regulates exosome microRNA enrichment in human cancer cells

Fig. 2

SRSF1 binds to miR-1246. a qRT-PCR detection of miR-1246 in IgG, GAPDH, SRSF1, EIF3B, and TIA1 immunoprecipitants of PANC-1 lysate (n = 3, *p < 0.001, student t-test). b-c EMSA detection of the SRSF1-miR-1246 complex (hot probe: IRD-800 labeled miR-1246 mimics; cold probe: miR-1246 mimics, n = 3). Direct binding of GST-SRSF1 and miR-1246 (b); and concentration-dependent competition between the cold and hot miR-1246 probe for binding to GST-SRSF1 (c). d Semi-quantification of SRSF1 and miR-1246 binding in C and calculated dissociation constant (n = 3)

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