Fig. 8
From: The deubiquitinase USP22 regulates PD-L1 degradation in human cancer cells
Inhibition of Usp22 suppresses tumorigenesis. a Cell surface PD-L1 level was measured after treatment with CTRL or USP22 siRNA in Calu-1 cell line. b Mean fluorescence intensity (MFI) was analyzed. Values are means ±SD, n = 3. Statistical differences were determined by two-sided Student’s t-test, **P < 0.01. c USP22, CSN5, PD-L1 protein level were assessed in pGIPZ-shluc and pGIPZ-shUSP22 infected B16-F10 cells. d B16-F10 tumor grow over time after transduction with the indicated lentiviruses. Tumor weight were measured every second day. Error bars represent SD (n = 5). e Tumor volume were assessed in (b). Error bars represent SD (n = 5). **P < 0.01, *P < 0.05, Statistical differences were determined by one-way ANOVA. f Representative images of the tumors in (c) taken 12 days after inoculation. g Tumor weight were measured. Error bars represent SD (n = 5). **P < 0.01, *P < 0.05, Statistical differences were determined by one-way ANOVA