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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The deubiquitinase USP22 regulates PD-L1 degradation in human cancer cells

Fig. 4

USP22 induces PD-L1 deubiquitination. a HEK293FT cells were transfected with indicated constructs. The impact of USP22 overexpression on PD-L1 ubiquitination were tested. b PD-L1 ubiquitination were conducted upon USP22 depletion. c Wild type or loss of function mutation (C185A) of USP22 were transfected to measure PD-L1 ubiquitination. e The effects of USP22 and HRD1 on PD-L1 ubiquitination. d USP22 was introduced to analyze glycosylated and non-glycosylated PD-L1 ubiquitination in HEK293FT cells. PD-L1-3NQ-FLAG means Substitution of each of the three asparagine (N) to glutamine (Q)— N192Q, N200Q, N219Q, which is critical for PD-L1 glycosylation

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