Fig. 2

USP22 influences PD-L1 protein stabilization. a A549, H1792, H1299 cells were transfected with control (CTRL) or USP22 siRNA for 24 h and then subjected to western blot analysis. b A549, H1792, H1299 cells were transfected with pcDNA3.1-USP22-HA plasmid for 24 h and then subjected to western blot analysis. pcDNA3.1 empty vector was transfected as control. c H1792 cells were transfected with control (CTRL) or USP22 siRNA for 24 h before RNA was extracted and subjected to reverse transcript PCR (RT-PCR). After agarose gel electrophoresis, mRNA levels were determined by image J. Error bars represent SD (n = 3), ***P < 0.001. d PD-L1 protein level was measured upon USP22 knockdown with or without proteasome inhibitor MG132 in H1792 cells. e H1299 cells were transfected with control (CTRL) or USP22 siRNA for 24 h followed by cycloheximide (CHX) (20 μg/ml) for indicated time. Relative protein abundance was measured by image J. Experiments were repeated three times. f H1299 cells were transfected with pcDNA3.1 empty vector or pcDNA3.1-USP22-HA plasmid for 24 h followed by cycloheximide (CHX) (20 μg/ml) for indicated time. Relative protein abundance was measured by image J. Experiments were repeated three times