Fig. 6

MDSCs activated by RDEs accelerated homologous tumor growth through immunosuppression of CTL. Three different tumor models were established using BALB/c mice subcutaneously injected with Renca, 4 T1 and CT26 cells. Each model was divided into three groups, treated with MDSCs^RED, MDSCs^PBS or PBS, respectively. a The tumor was stripped and showed after the mice were sacrificed on days 20 after subcutaneous tumor cells. b Analysis and compare of tumor volume every 2 days in different treatment. c Representative haematoxylin and eosin (H&E) staining of different group tumor model. d Representative Ki-67 immunohistochemical staining of different group (brown, Ki-67; blue, nuclei). e. CTLs infiltration in tumor tissues of various tumor models with different treatment by ISTS. Fluorescence images of tissue sections stained with CD8+ T in red and cell nuclei were labeled with DAPI (blue). All experiments were repeated at least three times and three mice were in each group