Fig. 4

T lymphocytes cytotoxicity were launched by the maturation of DCs. a Flow cytometry for analyzing levels of surface proteins in DCs pulsed with RenCa cell lysate, 4 T1 cell lysate, CT26 cell lysate or PBS (unpulsed control). b Experimental scheme was used for description the cytotoxicity of CD8 + T lymphocytes induced by DCs. c Cytolysis assay for CD8 + T lymphocytes activated by DCs^lys or DCs against autologous tumor cells at different E: T (effector: target) ratios. PBS was used as negative control. The data are representative of three independent experiments and expressed as the mean ± SD (*P < 0.05). DCs^lys, the group of tumor cell lysate-pulsed DCs; DCs, the group of unpulsed DCs; PBS, the group of PBS-stimulated CD8 + T cells. All experiments were repeated at least three times and three mice were in each group