Fig. 2

RDEs drived the expansion and activation of MDSCs in vivo. a Experimental scheme of induced and isolated MDSCs. Representative flow cytometry experiment showed the percentage of cells expressing Gr-1+ and CD11b + markers in BM cells (b) and speen (c) at days 10, 20 and 30 with the treat of PBS, RDEs or RenCa cells, and the statistical resultes were showed in (d and e). The production of ROS, NO and arginase activity, as well as IL-10 and TGF-β expression were measured in MDSCs isolated from BM (f) and spleen (g). All experiments were repeated at least three times and three mice were in each group