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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in mice

Fig. 2

Fstl1 was associated with liver fibrosis and the activation of hepatic stellate cells. a-c qRT-PCR analysis of α-SMA, Col1, Fstl1 mRNA expression in liver tissues of C57BL/6 mice at the indicated time points after CCl4 treatment (n = 6 per group). d Western blot analysis of Fstl1 protein in liver tissues of C57BL/6 J mice at the indicated time points after CCl4 treatment (n = 3 per group). β-Actin was used as a loading control. e Frozen liver sections from oil or CCl4 treated mice were stained with Fstl1 (red), α-SMA (green) and DAPI (blue). Scale bar: 25 μm. f LFQ (label-free protein quantification) intensity of Fstl1 in murine liver, hepatocyte, kupffer, cholangio, HSC and LSEC (liver sinusoidal endothelial cells) (n = 4 per group). g-i qRT-PCR analysis of α-SMA, Col1, Fstl1 mRNA expression in primary isolated mouse HSCs in in vitro culture at the indicated time points (n = 5 per group). Throughout, data represent mean ± SEM.* P < 0.05, **P < 0.01, ***P < 0.001

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