Fig. 2
High AMAP1 expression levels statistically correlate with fibrosis in human PDACs and KPC tumors. a Representative images of sirius red staining of clinical specimens, and Kaplan-Meier plots of overall survival of patients with regard to the staining. Bars = 200 μm. b Comparison of sirius red staining intensities in human samples with high or low AMAP1 expression or phosphorylated FAK (p-FAK) levels. c Representative images and quantification of sirius red and collagen I staining of tumors formed by control (Irr) or AMAP1-silenced (shAMAP1 #1 and #2) KPC cells in C57BL/6 mice. Error bars represent the mean ± s.e.m. Bars = 100 μm. d Representative IHC images of p-FAK and Kaplan-Meier plots with regard to the different levels of p-FAK in human PDACs. Bars = 50 μm. e Immunoblot analysis of FAK, p-FAK, and AMAP1 levels in control (Irr) and shFAK or shAMAP1 KPC cells, after culturing for 40 h in vitro. β-actin was used as a control. f A positive correlation between AMAP1 and p-FAK expression levels in human PDACs. *P < 0.05, **P < 0.01. P- values were obtained by the log-rank test (a and d) and by t-tests (b, c, and f)