Fig. 4

AKT/FOXO1 pathway inhibition amends the abnormal characteristics of TIE2-L914F mutant ECs. a, b BrdU assay was performed to study the proliferation of NC-ECs, TIE2-WT-ECs and TIE2-L914F-ECs treated with DMSO or rapamycin. c CCK-8 experiment was performed to study the effects of rapamycin on the cell activity of NC-ECs, TIE2-WT-ECs and TIE2-L914F-ECs. **P < 0.01 TIE-L914F versus TIE2-L914F + RAPA d, e Transwell assay was used to study the effects of rapamycin on the migration ability of NC-ECs, TIE2-WT-ECs and TIE2-L914F-ECs over a period of 24 h. f, g Apoptotic assay showed the apoptosis of NC-ECs, TIE2-WT-ECs and TIE2-L914F-ECs, and the effect of rapamycin on cellular apoptosis. h, i Tube formation assay was carried on NC-ECs, TIE2-WT-ECs and TIE2-L914F-ECs treated with DMSO of rapamycin. The total length and the number of branch points were used as indicators. j, k Expression of autophagy related indexes (p62, LC3I, LC3II) in different treated ECs was detected by western blot. Scale bars, 100 μm (a, d), 500 μm (h). The data are means ± SEM (n = 3). ns: no significant, *P < 0.05, **P < 0.01