Fig. 3

Effects of TIE2-L914F mutation on the biological characteristics of ECs. a, b 48 h after transfecting, ECs underwent BrdU assay. The ratio of positive cells to total cells in each visual field was analyzed by ImageJ software. c Different treated ECs underwent CCK-8 experiment, the OD values of 450 nm at day 1, 2, 3, 4 were tested. **P < 0.01 TIE-L914F versus NC, GFP and TIE2-WT. d, e Transwell assay was used to study the migration ability of NC-ECs, GFP-ECs, TIE2-WT-ECs and TIE2-L914F-ECs over a period of 24 h. f Tube formation assay of different groups of ECs. (up: inverted microscope; below: fluorescence microscope). g Branch points (left) and total length (right) of each group were measured. Scale bars, 100 μm (a), 200 μm (d), 500 μm (f). The data are means ± SEM (n = 3). ns: no significant, **P < 0.01