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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: AKT/FOXO1 axis links cross-talking of endothelial cell and pericyte in TIE2-mutated venous malformations

Fig. 2

TIE2-L914F mutation activates AKT/FOXO1 signaling. a ECs were transfected with lentivirus expressing GFP, TIE2-WT, TIE2-L914F respectively. Red arrows: typical elongated cell morphology of ECs. b ECs expressing lentivirus vectors under fluorescence microscopy. c Relative mRNA level of TIE-2. d, e 48 h after transfecting, the expression levels of AKT, p-AKT (ser473), FOXO1 and p-FOXO1 in ECs were then measured by Western blot. Flag tags were used to verify effectiveness of transfection. Scale bar, 200 μm. The data are means ± SEM (n = 3). ns: no significant, **P < 0.01

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