Fig. 6

c-MYC transcriptional activates FBXL6 in HCC. a The mRNA and protein levels of FBXL6 in SMMC-7721 cells or Hep3B cells transfected with EV or c-Myc plasmid were detected by real-time quantitative PCR and immunoblotting, respectively. b The mRNA and protein levels of FBXL6 in SMMC-7721 cells or Hep3B cells infected with the indicated shRNA lentiviruses were detected by real-time quantitative PCR and immunoblotting, respectively. c Proximal promoter region of human FBXL6 gene contains a potential binding site of c-Myc. The -3000 bp region from the TSS of FBXL6 was used to scan the potential binding sites of c-MYC. The top one sequence of the software provided was CACGTG, started at 551 and ended at 556. d Chromatin immunoprecipitation (ChIP) assays showing representative c-Myc binding to the FBXL6 promoter in Hep3B Cells. Cells were subjected to ChIP assays with anti-IgG or c-Myc antibodies. The promoter of GAPDH was used as negative control. e Luciferase reporter assays. HEK293T cells were co-transfected EV or c-Myc plasmids with luciferase reporter plasmids containing wild-type (WT-Luc) or mutant (Mut-Luc) binding site of c-Myc