Fig. 4

BP inhibits macrophage proinflammatory mediators and TF secretion by LPS stimulation. The Raw264.7 macrophage cell line was pretreated with BP for 2 h and then challenged with LPS (1 μg/ml) for 0.5 or 6 h. a, b CCK-8 assay was used to measure cell viability and proliferation (n = 6 per group; two-tailed Student’s test). c ELISA for IL-6, TNF-α, and TF in macrophage supernatant. d Laser confocal microscope screening of TF (green) and nuclei (blue) staining in macrophages (scale bar: 1 μm). e The mRNA expression of the indicated genes in macrophages was assayed by RT-PCR (n = 4 per group; results are the mean ± SEM; one-way ANOVA test). f The protein levels of JAK2/STAT3, NF-κB, MAPK and AKT signaling molecules in macrophages were assayed by immunoblotting (n = 3 per group). **P < 0.01 and *P < 0.05 (LPS versus DMSO); ##P < 0.01 and #P < 0.05 (LPS versus LPS + BP); ns indicates not significant