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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: miR-146a-5p impairs melanoma resistance to kinase inhibitors by targeting COX2 and regulating NFkB-mediated inflammatory mediators

Fig. 3

miR-146a spoils resistance of melanoma cells by repressing COX2 expression. a Silencing of COX2 by siRNA transfection (siCOX2) enhanced cell growth inhibition and increased cell cytotoxicity and apoptosis upon treatment with BRAF/MEKi. b Combined treatment with celecoxib increased cell growth inhibition and cytotoxicity by BRAF/MEKi. ●: interaction index = 1. c Increased apoptotic signaling after combination treatment with celecoxib and BRAF/MEKi. Phosphorylated and cleaved apoptotic factors showing increased expression in cells treated with the drug combination compared to BRAF/MEKi treated cells (Fold change > 1.3) by apoptosis signaling antibody array analysis. d Reduction of PGE2 release in culture media by treatment with the COX2 inhibitors celecoxib and NS398, alone or in combination with BRAF/MEKi. All experiments were carried with the LM47R cell line. P values were calculated by Student’s unpaired t test in B, and by one-way ANOVA followed by Bonferroni correction in D. *: p < 0.05, **: p < 0.01, ***: p < 0.0001

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Cell Communication and Signaling

ISSN: 1478-811X