Fig. 6

NEAT1 was downstream to TLR9-MYD88 and upstream to STAT3 in ADV-induced GSCs formation. a Primary glioma cells were infected with ADV and transfected with siRNAs to TLR9, and then the expression of NEAT1 was determined by RT-qPCR, with β-actin as a reference control. b Primary glioma cells were infected with ADV and transfected with siRNAs to MYD88, and then the expression of NEAT1 was determined by RT-qPCR, with β-actin as a reference control. c, d Primary glioma cells were infected with ADV and transfected with NEAT1 siRNAs, and then the level of STAT3 and p-STAT3 was determined by western blotting, with β-actin as a reference control (NC, negative control. 1, siRNA-1. 2, siRNA-2). Data are represented as mean ± SEM, n = 6. *, P < 0.05. **, P < 0.01. ***, P < 0.001