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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells

Fig. 1

Akt1Myr/KRasG12D mice show profound stromal activation and tissue remodeling with chronic inflammation. a Schematic of cerulein (CER) injection strategy shows that all genotypes were injected i.p. hourly for 5 h with PBS or 50 μg/kg CER bi-weekly for 3 months. Mice were euthanized 48 h (for histology) and 16 h (for flow cytometry) after the 4th and 8th injections (indicated by the red arrows). b Histology of pancreatic tissue for KRasG12D and Akt1Myr/KRasG12D mice injected with 4 rounds of CER injections (4-month-old) or 8 rounds of CER injections (6-month-old). Smooth muscle actin (α-SMA; brown) for activated PSCs near fibrotic regions. Trichrome staining for acinar tissue (red) & collagen-rich matrix (green-blue) in fibrotic areas. Periostin (brown) expression correlating to regions of activated PSCs and collagen. Images were acquired at 10x magnification and the scale bar denotes 100 μm. c Quantification of percent area of trichrome staining in Akt1Myr/KRasG12D and KRasG12D after 8 rounds of CER (n = 7 mice, 3 to 8 random fields were analyzed per mouse). d Akt1Myr/KRasG12D mice with chronic inflammation are prone to developing tumors that metastasize. H&E of Akt1Myr/KRasG12D pancreas tumors (T) and/or spleen (S) or small intestine (SI) metastasis after cerulein from PDAC diagnosed mice in Table S1

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