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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Remodelling of the bone marrow microenvironment by stromal hyaluronan modulates the malignancy of breast cancer cells

Fig. 5

HA promoted the growth of MDA-MB-231BO cells via the CD44 receptor. a. The knockdown effect of CD44 shRNA on MDA-MB-231BO cells was detected by western blots. The band intensities were analysed by densitometry analysis (****p < 0.0001 compared with the control shRNA group as measured by unpaired Student’s t test). b. MDA-MB-231BO-CD44 shRNA cells and MDA-MB-231BO-control shRNA cells were cocultured with HS5 cells for 72 h. The fluorescence intensity of GFP was measured. Data are presented as the mean ± SEM (****p < 0.0001 compared with the MDA-MB-231BO-control shRNA cells alone group as measured by unpaired Student’s t test, #### p < 0.0001 compared with the group of HS5 cells cocultured with MDA-MB-231BO-control shRNA cells as measured by unpaired Student’s t test). c. The knockout efficiency of CD44 in MDA-MB-231BO cells was detected by western blots. The band intensities were analysed by densitometry analysis (****p < 0.0001 compared with the control CRISPR/Cas9 group as measured by unpaired Student’s t test). d. MDA-MB-231BOCD44−/− cells and MDA-MB-231BO cells were injected into the tibia of mice, and the tibias were monitored on days 0, 11, 17, 24 and 31. n = 16 (p = 0.0482 compared with the MDA-MB-231BO-treated group by the Mantel-Cox test). e. The micro-CT images showed the bone destruction of the two groups at days 0, 11, 17, 24, and 31 after the injection. Arrows indicate the site of bone damage

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