Fig. 2

The BM stromal cell line HS5 promoted MDA-MB-231BO-GFP cell proliferation. a. The effect of GFP protein on the growth of MDA-MB-231BO cells was analysed by CCK-8 assays. b. The growth of MDA-MB-231BO-GFP cells after coculturing with HS5 stromal cells was evaluated by detecting the fluorescence intensity of GFP. The asterisk indicates that the relative growth of MDA-MB-231BO-GFP cells in the coculture environment was significantly promoted compared with that of the control group of MDA-MB-231BO-GFP cells alone. Bars represent the mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.005 and ****p < 0.0001 by unpaired Student’s t test). c. Fluorescence graphs show the number of MDA-MB-231BO-GFP cells after coculture with stromal HS5 cells or culture alone for 0 and 3 days. d. After coculture with HS5 cells for three days, the proliferation-related proteins PI3K, Cyclin D1, and CDK4 in MDA-MB-231BO-GFP cells were detected by western blots