Fig. 6
From: Phosphoproteomics of short-term hedgehog signaling in human medulloblastoma cells
Phosphorylation of HH pathway components after 15 min treatment. a SAG impairs GLI phosphorylation after 15 min stimulation. b Vismodegib binding induces delocalization of Smoothened in the primary cilium. c Heatmap of regulated and identified phosphosites after 15 min SAG and vismodegib treatment. Blue and red ellipses, phosphosites down- or upregulated compared to control. All quantified phosphopeptides of phosphoproteins after 15 min treatment assigned to sonic hedgehog signaling search were considered for the heatmap without applying any cutoff value. SAG/DMSO represents the ratio determined by the mean of the proteome-normalized intensities detected after SAG treatment (N = 3) divided by the mean of proteome-normalized intensities after DMSO (N = 3). Vismo/DMSO represents the ratio determined by the mean of proteome-normalized intensities detected after vismodegib treatment (N = 3) divided by the mean of proteome-normalized intensities after DMSO (N = 3). Significantly regulated phosphopeptides between SAG and vismodegib treatment are highlighted in bold