Fig. 4
From: Phosphoproteomics of short-term hedgehog signaling in human medulloblastoma cells
Volcano plot and kinase substrate enrichment analysis for phosphopeptides identified after 5.0 min treatment. a Distribution and significantly regulated phosphopeptides between SAG and vismodegib treatment. Ratios were determined by division of the ratio SAG/DMSO by the ratio vismodegib/DMSO: SAG/vismodegib. LIMMA statistical testing was applied to determine p-values and statistically significant regulated phosphopeptides. Orange and blue dots, significantly down- or upregulated phosphopeptides, respectively (threshold of p < 0.05.0); log2-fold-change cutoff was set to 0.5 and – 0.5 (b) and (c) Kinase Substrate Enrichment Analysis. Proteome normalized ratios of SAG/DMSO (b) and Vismo/DMSO (c) were taken to infer the kinase activation score with a p-value cutoff of 0.05. Significantly activated kinases are presented in red, significantly attenuated kinases in blue. A table with enriched kinases with corresponding numbers is attached in the supplementary material