Fig. 4

Prion peptide (106–126) treatment suppressed AMPK phosphorylation in neuronal cells. a Primary neurons were incubated with 50, 100, and 200 μM of PrP (106–126) for 6 h. Phospho-AMPK levels were analyzed by western blot analysis, indicate that PrP decreased AMPK phosphorylation. b Bar graph represents the mean levels of phospho-AMPK. c Immunocytochemistry to detect phospho-AMPK in SK-N-SH cells. d The bar graph represents the mean green fluorescence intensity. Results represent at least three independent experiments. Data are expressed as the mean ± SEM. ** p < 0.01, *** p < 0.001, compared to control (one-way ANOVA). PrP, Prion peptide (106–126); adj.volume, adjustment of volume (band volume minus background volume)