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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: AMPK activation overcomes anti-EGFR antibody resistance induced by KRAS mutation in colorectal cancer

Fig. 5

AMPK modulated the translation of Mcl-1 by targeting Mtor pathway. a The mRNA level of Mcl-1 in DLD1 WT and Kras mutated cells treated as indicated. b The expression of Mcl-1 in DLD1 Kras mutated cells treated with 5 μM Met and/or 5 μM MG132. c The expression of Mcl-1 in DLD1 Kras mutated cells treated with 5 μM Met and/or 1 ng/ml CHX. d The expression of indicated proteins in DLD1 WT and Kras mutated cells. e The expression of indicated proteins in DLD1 Kras mutated cells treated with 5 μM Met for indicated time. f The interaction of AMPK, TSC1, TSC2 in DLD1 Kras mutated cells treated with Met and/or Cet was analyzed by immunoprecipitation with anti-TSC2 antibody. g The expression of indicated proteins in DLD1 Kras mutated cells transfected with TSC2 siRNA and treated with Met and Cet. (H) The apoptosis in DLD1 Kras mutated cells treated as in (g). Each experiment was repeated for 3 times. **, p < 0.01

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Cell Communication and Signaling

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