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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Heat shock response regulates stimulus-specificity and sensitivity of the pro-inflammatory NF-κB signalling

Fig. 5

NF-κB responses exhibit cytokine-specific temperature sensitivity. a Schematic representation of the differential temperature treatment. b Schematic representation of the HS treatment protocol: cells exposed to 1 h 38–43 °C HS range and subjected to cytokine stimulation following different recovery time. c Model simulations of the TNFα-induced NF-κB responses following 1 h temperature exposure and different recovery times. Shown are average nuclear NF-κB trajectories (based on 1000 simulated cells, in number of molecules) for the 38–43 °C temperature range and normal 37 °C conditions (in diffrent coloured lines); the right 0–6 h axis represents time from the end of HS, on the left: no HS control. d Model simulations of the IL1β-induced responses as described in c. e Nuclear NF-κB trajectories in MCF7 cells stably expressing p65-EGFP in response to TNFα stimulation. Cells were stimulated with TNFα in normal temperature 37 °C (left, data from Fig. 1), immediately after 1 h 41 °C exposure (middle), or after 4 h recovery (right). Top: individual single cell nuclear NF-κB trajectories (n = 50 per condition, in aribirary fluorescene units) depicted with coloured lines (green 37 °C, yellow 41 °C); black lines represented the population averages. Bottom: heat maps of trajectories normalized across all conditions in e and f (represented on a 0–3 scale). Cells monitored for up to 10 h from the beginning of cytokine stimulation. f Characteristics of TNFα-induced responses from e. From the left: distribution of area under the curve (AUC), first peak amplitude, and time to first response. Individual cell data are depicted with circles (with mean ± SD per condition). Kruskal-Wallis one-way ANOVA with Dunn’s multiple comparisons test was used to assess differences between groups (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns – not significant). g Nuclear NF-κB trajectories in MCF7 cells stably expressing p65-EGFP following treatment with IL1β, represented as in e. p65-EGFP trajectories of cells cultured in normal conditions taken from Fig. 2. h Characteristics of IL1β-induced responses from g, data are represented as in f

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