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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Upregulation of fibronectin following loss of p53 function is a poor prognostic factor in ovarian carcinoma with a unique immunophenotype

Fig. 6

Relationship between FN and cell kinetics. a Upper: phase-contrast images of OVISE cells treated with 20 μg/ml FN for 96 h. Note there was no alteration in cell morphology during the treatment. Lower: western blot analysis for the indicated proteins in total lysates from OVISE cells with or without 20 μg/ml FN treatment. b Western blot analysis of the indicated proteins in total lysates from OVISE cells with or without 10 μM CDDP treatment in the presence or the absence of 20 μg/ml FN. c Left: wound-healing assay with OVISE cells with or without 20 μg/ml FN treatment. A scratch ‘wound’ was made in the middle of cells grown to confluency, and phase contrast images were taken after 6, 9, and 12 h. Right: the values of wound areas in 0 h were set as 1. The fold changes in wound areas are presented as means±SDs. d Migration rate measured using the transwell assay. Left: the OVISE cells with or without 20 μg/ml FN treatment were seeded in 24-well transwell plates and incubated for 24 h in medium without serum. The cells were stained by HE and counted using light microscope. Right: numbers of migrated cells are presented as means±SDs. e The OVISE cells with or without 20 μg/ml FN treatment were seeded at low density. The cell numbers are presented as means±SDs. P0, P3, P6, and P8 are 0, 3, 6, and 8 days after cell seeding, respectively

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