Fig. 5

Relationship between p53 and FN expression. a Unsupervised hierarchical clustering of mRNA expression detected by next generation sequencing in OV-shp53 and control cells (Con). The expression level of each mRNA is colored; red, black, and green indicated high (> 4), neutral (1–4), and low (< 1), respectively. Major clusters are shown as group I to XI. b Analysis of endogenous FN1 mRNA expression by conventional (left) and real time RT-PCR assay (right) in OV-shp53 and control cells (Con). The values of endogenous FN1 mRNA expression detected by conventional RT-PCR assay were normalization to GAPDH. The fold changes in mRNA expression for both assays are presented as means±SDs. The experiment was performed in triplicate. c Western blot analysis for the indicated proteins in total lysates from OV-shp53 and control cells (Con). d OVISE cells were transfected with FN1 reporter constructs, together with either p53wt or p53mt. Relative activity was determined based on arbitrary luciferase light units normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means±SDs. The experiment was performed in duplicate