Fig. 2

Relationship between cell phenotypic characteristics and p53-KD. a Left: phase contrast images of OV-shp53 cells. Note the changes in cell morphology toward fibroblastic appearances in OV-shp53 cells. Right: the numbers of spindle-shaped cells are presented as means±SDs. b Western blot analysis for the indicated proteins in total lysates from OV-shp53 and control cells (Con). c OVISE cells were transfected with Snail reporter constructs, together with either p53wt or p53mt. Relative activity was determined based on arbitrary luciferase light units normalized to pRL-TK activity. The activities of the reporter plus the effector relative to that of the reporter plus empty vector are shown as means±SDs. The experiment was performed in duplicate. d Analysis of endogenous Snail mRNA expression by conventional (left) and real time RT-PCR assay (right) for OV-shp53 and control cells (Con). The signals of endogenous Snail mRNA expression in the conventional RT-PCR assay were normalized to GAPDH. The fold changes in mRNA expression detected by both assays are presented as means±SDs. The experiment was performed in triplicate