Fig. 4

Expression of inflammatory factors in adult brains. a qRT-PCR. Adult WT or KI male mice were treated with intraperitoneally injected LPS (5 mg/kg, ip.) or saline for each group for 2 h, from the whole brains of which RNAs were prepared for subsequent analysis. Assay was duplicated for each RNA sample. With 6 RT-PCR data for either WT or KI, Two Way ANOVA plus post hoc test with Bonferroni’s multiple comparisons test (Version 5.0, Stat view-j) was used for statistical analysis. Values are presented as means ± S.D.. *, p < 0.05, n = 3. b Cytokine antibody arrays. From the same brains used in (a), whole extracts were prepared, 300 μg proteins of which were subjected to analysis. c Mean Pixel Densities were obtained from each spot using Image J soft