Fig. 3
From: Estrogen receptor α phosphorylated at Ser216 confers inflammatory function to mouse microglia
Microglia in substantia nigra of adult brains. a Saline or LPS (5 mg/kg, ip., 2 h)-treated brains. Brains were removed from 6-month-old ERα WT and KI males, from which frozen sections were prepared for immunostaining with an anti Iba-1 antibody. The brain region showing the microglia morphological changes is substantia nigra (SN). b Three brains were used for each group. Four sections from each brain were stained for analysis. Stained microglia were scanned to semi-quantitate microglia activation. Values are presented as means ± S.E., by taking values of ERα WT as 100%. Statistical analysis was conducted with One Way ANOVA plus post hoc test with Bonferroni’s multiple comparisons test (Version 7.00, GraphPad, San Diego, CA). **, p < 0.01