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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: CAMKK2-CAMK4 signaling regulates transferrin trafficking, turnover, and iron homeostasis

Fig. 8

TF-signaling mediated intracellular Ca2+ release in CAMKK2−/−, CAMK4−/−, and DKO HEK293 cells. a: Line graphs showing temporal alterations of Fluo-4 intensity change ([Ca2+]i) in the wild-type, CAMKK2−/−, CAMK4−/−, and DKO HEK293 cells following treatment with 25 μM TF. The confocal image acquisition parameters and the treatment condition between different experimental sets were kept uniform and therefore, difference in the relative intensity was comparable. b: Scatter plot showing basal [Ca2+]i. N = 40–50 cells analyzed in 5 independent experiments in each category. P values by one-way ANOVA followed by Dunnett’s multiple comparison test. The entire dataset is presented in Fig. S7. c-d: Scatter plots showing the mean Fluo-4 intensity after 2 mins (c) and 9 mins (d) of TF treatment. N = 40–50 cells analyzed in 5 independent experiments in each category. The entire dataset is presented in Figure S7. P values by one-way ANOVA followed by Dunnett’s multiple comparison test

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