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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: CAMKK2-CAMK4 signaling regulates transferrin trafficking, turnover, and iron homeostasis

Fig. 7

CAMKK2-CAMK4 signaling regulates signal transduction-mediated Ca2+ release from the ER. a, d-f: Line graphs showing temporal alterations of Fluo-4 intensity change ([Ca2+]i) in the wild-type, ΔGsix0, CAMKK2−/−, CAMK4−/−, and DKO HEK293 cells treated with 10 μM muscarine chloride. The confocal image acquisition parameters and the treatment condition between different experimental sets were kept uniform; therefore, the relative intensity difference was comparable. At the beginning of the time-lapse imaging, the mean Fluo-4 intensity per cell was set at a threshold below 50 pixel intensity (arbitrary unit) to capture the dynamic range of muscarine-induced Ca2+ release in different cell types irrespective of the cells basal [Ca2+]i level. (b-c & g): Scatter plots showing the mean Fluo-4 intensity. N = 40–50 cells analyzed in 5 independent experiments in each category. P values by one-way ANOVA followed by Dunnett’s multiple comparison test. H: Scatter plot showing basal [Ca2+]i. The basal [Ca2+]i in the wild-type, CAMKK2−/−, CAMK4−/−, and DKO HEK293 cells was measured in a different experiment where no initial threshold was set as mentioned previously, however, the microscopic image capture settings were kept uniform. N = 40–50 cells analyzed in 5 independent experiments in each category. P values by one-way ANOVA followed by Dunnett’s multiple comparison test. The entire dataset is presented in Supplementary Fig. S7

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