Fig. 2
From: CAMKK2-CAMK4 signaling regulates transferrin trafficking, turnover, and iron homeostasis
Loss of CAMKK2 and/or CAMK4 significantly increased TF-uptake by the HEK293 cells. a-e: Immunoblot showing relative expression/abundance of RFP-TFRC (a), TF(b-c), CAMKK2 (d), CAMK4(e) in the wild-type, CAMKK2−/−, CAMK4−/−, and DKO HEK293 cells treated with 25 μg/ml partially iron-saturated human TF. f: Oriole-stained gel showing total protein loading. g: Scatter plot showing the relative amount of TF. N = 6, 2 replicates from three independent experiments. P values by one-way ANOVA test followed by multiple comparisons.