Fig. 4
From: MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function
MKL-1TAD domain interacts with STAT5b coiled-coil domain. a and b Domain structure of MKL-1 and STAT5b. STAT5b interacts with MKL-1from CD3+T cells transfected with mammalian Flag-tagged STAT5b and Myc-tagged MKL-1 (1–705) (MKL-1△TAD). MKL-1 interacts with STAT5b from CD3+T cells transfected with mammalian Myc-tagged MKL-1 and Flag-tagged STAT5b (145–787) (M1-STAT5b) or Flag-tagged STAT5b (331–787) (M2-STAT5b); c The predicted STAT5b-binding site (MKL-1 TAD) is shown in A. This latter region was deleted to generate MKL-1 TAD domain mutant. Coimmunoprecipitation shows decreased association of STAT5b to MKL-1 TAD compared with WT-MKL-1; d The predicted MKL-1-binding site (STAT5b coiled-coil) is shown in A. This latter region was deleted to generate STAT5b coiled-coil domain mutant. Coimmunoprecipitation shows decreased association of MKL-1 to STAT5b coiled-coil compared with WT-STAT5b; e and f Luciferase assay detect expression of Foxp3 in HEK-293 T cells transfected with mutantion the coiled-coile domain of STAT5b and MKL-1 or the TAD domain of MKL-1 and STAT5b for 48 h. Results are normalized to the control or expressed as a percentage of the maximal effect of the given MKL-1 or STAT5b construct. **p < 0.01, *p < 0.05. Error bars indicate mean ± SEM