Fig. 3

Biological activities of FHF1. a Impact of FHF1 and FGF1 on cell proliferation in NIH3T3 and BaF3-R1c cells. The data shown are mean values ± SD of three independent experiments, presented as a percentage of maximal response. b Effect of FHF1 and FGF1 on glucose uptake in 3T3-L1 adipocytes. The data shown are mean values ± SD of three independent experiments expressed as a percentage of glucose uptake induced by serum. Student t-test was applied for statistical analysis; * p < 0.05, ** p < 0.01, n.s. – not significant. c Anti-apoptotic properties of FHF1 and FGF1 assessed by measurements of cell viability upon induction of apoptosis with 1 μM staurosporine in U2OS-R1 cells. The data shown are mean values ± SD of three independent experiments expressed as a percentage of viability of untreated cells; * p < 0.05, ** p < 0.01, *** p < 0.001. d Ratio of caspase 3/7 activity in serum-starved NIH3T3 cells stimulated with FHF1 and FGF1 to cell viability. The data shown are mean values ±SD of three independent experiments normalized toward untreated cells (relative caspase-3/7 activity). Student t-test was applied for statistical analysis; ** p < 0.01, *** p < 0.001. e PARP and caspase 3 cleavage in U2OS-R1 cells upon induction of apoptosis with 1 μM staurosporine and FHF1 or FGF1 treatment. f Long-term stability of FHF1 in cell conditioned media. FHF1 and FGF1 were incubated with NIH3T3 cells for 48 h, and then media containing recombinant proteins were aspirated and tested for their FGFR stimulatory activity with western blotting. Freshly prepared solution of recombinant FHF1 and FGF1 were used as controls