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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner

Fig. 2

FHF1 directly interacts with FGFRs. a ELISA analysis of binding of FHF1 and FGF1 to extracellular domains of FGFRs. Mean values of five independent experiments ± SEM are shown. Student t-test was applied for statistical analysis; ** p < 0.01, *** p < 0.001. b Scheme of SPR experiments using sensor chip with immobilized extracellular part of FGF receptors fused to the Fc fragment. c SPR analyses of FHF1 interaction with FGFRs immobilized at high density on CM5 sensor (9000 RU). d Kinetics of FHF1-FGFR1 interaction assessed with SPR. The FHF1 protein at the concentrations from 0.1 μM to 3.2 μM was injected on CM4 sensor surface with FGFR1-Fc immobilized at low density (1000 RU). Equilibrium dissociation constant (KD) was calculated from saturation binding curve. e Soluble extracellular domains of all FGFRs block the activation of FGFR by FHF1 in NIH3T3 cells. Cells were pre-incubated with FGFR1–4 fused to Fc fragment and the ability of FHF1 and FGF1 to trigger cell response was assessed with western blotting

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