Skip to main content
Fig. 1 | Cell Communication and Signaling

Fig. 1

From: FHF1 is a bona fide fibroblast growth factor that activates cellular signaling in FGFR-dependent manner

Fig. 1

Extracellular FHF1 activates FGFR1. a SDS-PAGE, b western blotting and c mass spectrometry analysis of recombinant FHF1 produced in bacterial expression system (calculated mass 24,302 Da). d CD spectrum of FHF1 between 205 and 260 nm. e Thermal denaturation curve of FHF1. f and g Activation of FGFR and downstream signaling by FHF1 and FGF1 in NIH3T3 (f) and U2OS-R1 (g) cells in the absence and presence of FGFR specific inhibitor (100 nM PD173074) assessed with western blotting. h Concentration-dependent activation of FGFR1 and ERKs cascades in NIH3T3 cells by FHF1 and FGF1 verified as in (f) and (g)

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X