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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: CLPTM1L induces estrogen receptor β signaling-mediated radioresistance in non-small cell lung cancer cells

Fig. 6

CLPTM1L directly interacts with and coactivates ERβ. a Model showing the effect of mutation of the LXXLL motif on abolishing the interaction between CLPTM1L and ERβ. b Confocal microscopy images of the localization of Flag-CLPTM1L (wt or mut) and ERβ in A549 and H460 cells exposed to 4 Gy IR. c The interaction of CLPTM1L (wt or mut) with ERβ was detected by Co-IP assays in A549 cells exposed to 4 Gy IR. d The direct interaction of recombinant GST-CLPTM1L (wt or mut) with His-ERβ was detected by GST pull-down assays and western blot analysis. ef CLPTM1L binding to the promoters of CDC25A, c-Jun, and BCL2 was examined by ChIP-qPCR in A549 cells exposed to 4 Gy IR. g Luciferase activities of Gal4-ERβ were measured by luciferase reporter gene assays in A549 cells exposed to 4 Gy IR. NS, not significant. Each experiment was repeated at least three times. Student’s t test; * P < 0.05; ** P < 0.01

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