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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: CLPTM1L induces estrogen receptor β signaling-mediated radioresistance in non-small cell lung cancer cells

Fig. 4

CLPTM1L upregulates the levels of CDC25A, c-Jun, and BCL2 through ERβ in NSCLC cells. a Real-time PCR analysis of the mRNA levels of CLPTM1L, CDC25A, c-Jun, and BCL2 in A549 cells exposed to different doses of γ-ray IR. b Western blot analysis of the protein levels of these genes in A549 cells. c Western blot analysis of the protein levels of these genes in H460 cells exposed to 4 Gy IR. d–e Real-time PCR and western blot analysis of the mRNA and protein levels of CLPTM1L, ERβ, CDC25A, c-Jun, and BCL2 in A549 cells treated with IR and siRNAs. f–g Real-time PCR and western blot analysis of the expression of these genes in H460 cells treated with the indicated plasmids/siRNAs and 4 Gy IR. All experiments were repeated at least three times. Statistically significant differences are indicated. Student’s t test; * P < 0.05; ** P < 0.01

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