Fig. 1

CLPTM1L induces the radioresistance of NSCLC cells. a The cell viabilities of different NSCLC cell lines exposed to 4 Gy of γ-ray irradiation (IR) were examined by colony formation assay after 12 days of IR and expressed as percent change compared with the control group (group without IR). b The expression levels of CLPTM1L in different NSCLC cell lines exposed to 4 Gy of IR were determined by western blot and real-time PCR analysis and expressed as fold change compared with the H460 group. c Heatmap showing the relationship between the expression level of CLPTM1L and the viability of NSCLC cells exposed to IR. d–e The expression levels of CLPTM1L in A549 and H460 cells exposed to various doses of γ-ray IR were detected by real-time PCR and western blot analysis. f The effect of CLPTM1L siRNA and/or 4 Gy of γ-ray IR on cell proliferation was measured by EdU assay in A549 cells. g The effect of CLPTM1L overexpression and/or 4 Gy of IR on cell proliferation was tested by EdU assay in H460 cells. h–i Cells (A549 and H460) pretreated with CLPTM1L siRNA or pcDNA-CLPTM1L (24 h) were exposed to IR (0, 2, 4, and 8 Gy). After 12 days, colonies were counted for quantification. Data are representative of three independent experiments; Student’s t test; * P < 0.05; ** P < 0.01; *** P < 0.001