Fig. 2

Plasmodium infection suppressed neovascularization in Hepa1–6-implanted tumor tissue. a-c The vascularization of tumor tissue from tumor-bearing mice on day 17 after Plasmodium infection was imaged (n = 4) (a). H&E staining showed neovascularization of the tumor in tumor-bearing mice on day 17 after Plasmodium infection (n = 4) (b, upper panel). Representative images (b, bottom panel) and quantitative analysis (c) of the microvessel density (MVD) by immunohistochemical staining with antibodies reactive to CD31 were used to characterize the degree of neovascularization in tumor-bearing mice on day 17 after Plasmodium infection. Original magnification: 400×. ***, p < 0.001. d-e The alginate-encapsulated tumor cell assay was performed as described in the “Materials and methods” section (n = 10). Representative images of the alginate beads are shown (d). The FITC-dextran uptake by the beads was quantified (e). The data are expressed as the means ± SDs. ***, p < 0.001