Fig. 3
From: MACC1 driven alterations in cellular biomechanics facilitate cell motility in glioblastoma
Effect of MACC1 overexpression on adhesion. a-f mRNA expression of CD44, fibronectin, integrin α5, β1, N-cadherin, laminin receptor 1 of U251 and U138 cells with and without overexpression of MACC1. No consistent MACC1 associated effect was observed. n = 3. g Illustration of a typical retract curve when using a coated cantilever. Arrowheads show discrete rupture events used for quantification. h Mean number of discrete adhesion events between U251 or U138 cells and laminin or fibronectin coated cantilevers. No MACC1 dependent effect was observed. The following numbers of cells were measured in three independent experiments: nU138/EV FN = 40, nU138/EV LN = 35, nU138/MACC1 FN = 40, nU138/MACC1 LN = 35, nU251/EV FN = 30, nU251/EV LN = 33, nU251/MACC1 FN = 35 and nU251/MACC1 LN = 35. Stars depict p < 0.05. Error bars depict the standard error of the mean