Fig. 2
From: β-Catenin and TCFs/LEF signaling discordantly regulate IL-6 expression in astrocytes
TCFs/LEF positively regulate IL-6 expression in normal human astrocytes. a NHAs were transfected with TCF1, TCF3, TCF4, LEF1, or scrambled (scrm) siRNA and at 48 h, TCF1, TCF3, TCF4, and LEF1 mRNA level measured by real-time PCR and data normalized to GAPDH. b NHAs were KD for TCF/LEF transcription factors (TFs) and IL-6 mRNA measured at 48 h. by real-time PCR and normalized to GAPDH. c NHAs were transfected with TCF1, TCF3, TCF4, LEF1, or pcDNA (control) expression plasmids and at 24 h IL-6 mRNA expression was measured by real-time PCR. * indicates p ≤ 0.05 in comparison to control. The data is presented as mean ± SEM (n = ≥ 3; in (a) and (b), one-way ANOVA with post hoc Dunnett’s test was performed and in c one sample T-Test was performed)