Fig. 6

TNFα/TNFR2 signaling pathway can regulate anti-inflammatory cytokine production by ECFCs. a A representative of the gating strategy for selecting CD31⁺CD144⁺KDR⁺ ECFCs. b A representative of flow cytometry analysis depicting ECFC’s capacity to produce anti-inflammatory cytokines TGFβ, IL-10 and HLA-G. c CB-ECFCs were treated either with TNFα or with anti-TNFR2 neutralizing mAb 1 day prior TNFα addition and their capacity to produce TGFβ, IL-10 and HLA-G production were compared with untreated ECFCs (basal level). Each dot represents a measured value (n = 6) collected from 2 independent experiments. For each group of values, horizontal lines represent mean value and standard error of the mean. One way ANOVA analysis was performed to generate P values. ns: non-significant, *P < .05, **P < .01, ***P < .001. KDR = CD309 or VEGF receptor type 2, ISO = Isotype control