Fig. 2

ECFCs can modulate CD4⁺ T cell activation markers. Anti-CD3/CD28 activated CD3⁺CD25⁻ effector T cells were co-cultured with HAECs, CB-ECFCs and APB-ECFCs in fixed 1/6 ratio. After 3 days, T cells were collected and different activation markers were studied. Cells were gated on CD4⁺Foxp3⁻ conventional T cells. For each marker the strategy of gating is indicated on the left and below the figure. The representative gating panels on the left demonstrate fluorescence minus one (FMO) controls. Each dot represents a measured value (n = 7 for Tcells alone, Tcells alone in RPMI+EGM2 media and n = 6 for Tcells + HAECs, Tcells + CB-ECFCs and n = 5 for Tcells + APB-ECFCs) collected from 2 independent experiments. For each group of values, horizontal lines represent mean value and standard error of the mean. MFI values have been normalized with T cells alone control group. One way ANOVA analysis was performed to generate P values. ns: non-significant, *P < .05, **P < .01, ***P < .001