Fig. 1

ECFCs can suppress T cell proliferation. Activated CFSE⁺CD3⁺CD25⁻ effector T cells (responder cells) were co-cultured with (a) HAECs, (b) APB-ECFCs and (c) CB-ECFCs in different ECs/T cells ratios (n = 8). Proliferation of CD4⁺ T cells (upper graphs) and CD8⁺ T cells (lower graphs) was measured by flow cytometry. The first bar represents the unstimulated T cells alone (n = 8), the second bar represents the anti-CD3/CD28 stimulated T cells alone in RPMI medium (n = 8), while the third bar is the anti-CD3/CD28 stimulated T cells alone in 50% RPMI+ 50% EGM2 media (n = 8). Data are represented as mean value ± SEM collected from 3 independent experiments. One way ANOVA analysis was performed to generate P values. ns: non-significant, *P < .05, **P < .01, ***P < .001. Stim: Anti-CD3/CD28 activation Beads